响应曲面法优化高剪切分散乳化提取破布木果多糖

刘晓光, 吴慧敏, 王冲, 于水, 吴娟, 李娇, 张洁 , 谢建伟

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石河子大学学报 ›› 2017, Vol. 35 ›› Issue (6) : 680-686. DOI: 10.13880/j.cnki.65- 1174/n.2017.06.004
化工·材料·食品

响应曲面法优化高剪切分散乳化提取破布木果多糖

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Study on high- speed shear treatment for extraction of polysaccharides fr om Cor dia dichotoma fr uits via response sur face optimization

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摘要

多糖 类 物 质 不 仅 是 一 种 非 特 异 性 免 疫 增 强 剂,而 且 具 有 降 血 糖、降 血 脂、抗 凝 血 等 多 种 生 理 活 性,本 文 探 究 了 维 药 破 布 木 果 多 糖 的 最 佳 提 取 工 艺。 采 用 高 剪 切 分 散 乳 化( High shear dispersing emulsification,HSDE) 技术,探 讨 了 液 料 比、高 剪 切 时 间、高 剪 切 速 度 对 多 糖 得 率 的 影 响,采 用 响 应 曲 面 法( Response Surface Method,RSM) 进行 优 化,得 到 最 佳 提 取 工 艺 参 数。 结 果 表 明:在 液 料 比 为 50∶1( mL∶g) ,剪 切 时 间 为 3.0 min,剪切 速 度 为 16000 r/min,破布 木 果 多 糖 得 率 为( 111.43±0.1) mg/g;高剪 切 分 散 乳 化 技 术 大 大 缩 短 了 提 取 时 间,避 免 有 效 成 分 的 损 失,在 天 然 产 物 提 取 方 面 极 具 应 用 前 景。

Abstract

Polysaccharide is not only a non- specific immune enhancement agent, but also has hypoglycemic, hypolipidemic, anticoagulant and other physiological activities. To study and optimize the best extract conditions of polysaccharides from Cordia dichotoma fruits, by using high shear dispersing emulsification(HSDE).The effects of treatment time, rotary speed and liquid- solid ratio were investigated. Based on the single- factor tests, response surface method(RSM) was used to optimize the extraction. The optimum HSDE treatment conditions were as follows:3 min of treatment time, 16000 r/min rotary speed and 50 mL/g of liquid- solid ratio, the contents of polysaccharides were 111.43±0.1 mg/g. The results indicate that the HSDE can provide a high contents in a short time and the method was easy and efficient. In conclusion, high shear dispersing emulsification save extraction time and avoid the loss of activity principle, it has great application prospect in natural product extraction.

关键词

破 布 木 果 / 多 糖 / 高 剪 切 分 散 乳 化 技 术 / 响 应 曲 面 法

Key words

Cordia dichotoma fruits / polysaccharides / HSDE / RSM

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刘晓光, 吴慧敏, 王冲, 于水, 吴娟, 李娇, 张洁 , 谢建伟. 响应曲面法优化高剪切分散乳化提取破布木果多糖. 石河子大学学报. 2017, 35(6): 680-686 https://doi.org/10.13880/j.cnki.65- 1174/n.2017.06.004
Liu Xiaoguang, Wu Huimin, Wang Chong, Yu Shui, Wu Juan, Li Jiao, Zhang Jie, Xie Jianwei. Study on high- speed shear treatment for extraction of polysaccharides fr om Cor dia dichotoma fr uits via response sur face optimization. Journal of Shihezi University. 2017, 35(6): 680-686 https://doi.org/10.13880/j.cnki.65- 1174/n.2017.06.004

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国 家 自 然 科 学 基 金 项 目( 81360453,81260412)
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